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NADP+ 2-oxo-3-methylvalerate NADPH CO2 (S)-2-aceto-2-hydroxybutanoate L-glutamate 2-oxoglutarate (R)-2,3-dihydroxy-3-methylvalerate pyruvate L-threonine NH3 2-oxobutanoate L-isoleucine 4.2.1.9 | dihydroxy-acid dehydratase  (isoleucine synthesis) 4.3.1.19 | threonine ammonia-lyase 1.1.1.86 | ketol-acid reductoisomerase  (isoleucine synthesis) 2.2.1.6 | acetolactate synthase (isoleucine synthesis) 2.6.1.42 | branched-chain-amino-acid transaminase  (isoleucine synthesis)