BP | Barke | pooled sample of 15 vegetative and generative barley tissues. PCR depleted against a ~20.000 unigene set and normalized. Manufactured by company GATC. | 7 | 7 | 688 | 7 | 0 | |
GAN | Ingrid WT | leaf epidermis; seedlings were grown at 18 degr. C, 60 % rel. humidity, and a photoperiod of 16 h (100 microE * s-1 * m-2). A. 8 days, seedl. were treated with ASM (syn. BTH; 20 ppm soil drench). Leaf epid. was harv. 8, 24, 48, 72 hpt | 3068 | 3068 | 403 | 2020 | 1048 | |
GBN | backcross: Ingrid mlo5 and Ingrid MLG | leaf epidermis; seedlings were grown at 18 degr. C, 60 % rel. humidity, and a photoperiod of 16 h (100 microE * s-1 * m-2). A. 8 days, seedl. were treated with cuture filtrate of Bipolaris sorokiniana. Leaf epid. was harv. 6, 12, 24, 48 hpt | 3058 | 3058 | 377 | 2325 | 733 | |
GCA | Chevron (resistent cultivar) | with Fusarium graminearum infected spikelets | 1286 | 1286 | 370 | 0 | 1286 | |
GCN | Chevron (resistent cultivar) | with Fusarium graminearum infected spikelets | 1486 | 1486 | 580 | 1486 | 0 | |
GCW | Carola , highly susceptible | roots, Seedlings were grown in a mixture of SERAMIS and OIL DRI soil at 20 degree C, 16h light and 50-60% rel. humidity for 7 days, Roots were harvested 6, 24, 48, 72, and 96 hpi (hours post inoculation) with Fusarium culmorum KF 350 | 1138 | 1138 | 374 | 0 | 1138 | |
GD | Ingrid MLG | leaf epidermis; seedlings were grown at 18 degr. C, 60 % rel. humidity, and a photoperiod of 16 h (100 microE * s-1 * m-2). A. 8 days, seedl. were inoculated with Blumeria graminis f.sp. tritici. Leaf epid. was harv. 3, 6, 10, 18, 24, 48 hpi | 78 | 78 | 313 | 78 | 0 | |
GNW | Nickel, susceptible | roots, Seedlings were grown in a mixture of SERAMIS and OIL DRI soil at 20 degr. C, 16h light and 50-60% rel. humidity for 7 days, Roots were harvested 6, 24, 48, 72, and 96 hpi (hours post inoculation) with Fusarium culmorum KF 350 | 1168 | 1168 | 366 | 0 | 1168 | |
GPN | Pasadena (susceptible cultivar) | with Fusarium graminearum infected spikelets | 1503 | 1503 | 495 | 1503 | 0 | |
GW | Nickel and Carola | roots (Control); Kernels were germinated and subsequently grown in a mixture of SERAMIS and OIL DRI soil at 20 degr. C, 16h light and 50-60% rel. humidity, Roots were harvested after 7 days | 847 | 847 | 437 | 0 | 847 | |
HA | Barke | embryosac, 0-7 DAF (days after flowering), filial tissue | 10055 | 12785 | 487 | 3022 | 9763 | |
HB | Barke | developing caryopsis, 8-15 DAF (days after flowering) | 10063 | 10275 | 472 | 245 | 10030 | |
HC | Sloop | coleoptile, 3 day | 5118 | 8169 | 514 | 3328 | 4841 | |
HD | Golden Promise | callus, 5-10mm in diameter | 4972 | 7418 | 515 | 2968 | 4450 | |
HDP | Barke | pooled sample of 15 vegetative and generative barley tissues. PCR depleted against a ~20.000 unigene set and normalized. Manufactured by company GATC. | 15258 | 29397 | 546 | 14588 | 14809 | |
HE | Barke | etiolated leaves (grown for 6 days in growth chamber, harvested at daylight conditions) | 336 | 551 | 427 | 242 | 309 | |
HF | Barke | developing caryopsis, 16-25 DAF (days after flowering) | 7420 | 7692 | 543 | 280 | 7412 | |
HG | Barke | green leaves | 344 | 565 | 467 | 237 | 328 | |
HH | Sloop | coleoptile, 1 day | 2397 | 3902 | 532 | 1856 | 2046 | |
HI | Barke | female inflorescences (approx. 3mm in size) | 4526 | 6985 | 535 | 2835 | 4150 | |
HJ | Sloop | embryos isolated 8 days after pollination, corresponding to the globular stage of embryo development. | 197 | 197 | 533 | 0 | 197 | |
HK | Barke | etiolated leafs. Plants were grown on filterpaper for 6 d at 250C in the dark. Leafs were harvested at daylight conditions | 1031 | 1383 | 480 | 891 | 492 | |
HL | Barke | RNA pool: embryo, scutellum and aleuron from seeds 12 h and 62 h after imbibition, respectively | 697 | 697 | 558 | 0 | 697 | |
HM | Barke | male inflorescences, anther approx. 2 mm in size (green anther stage) | 4740 | 7260 | 519 | 3023 | 4237 | |
HO | Ingrid BC mlo5 | Epidermis stripped 6 and 24 hours post inoculation from seven day old plants inoculated with Blumeria graminis hordei or B. g. tritici | 13143 | 22827 | 562 | 10711 | 12116 | |
HP | Barke | epidermis (seedlings grown in greenhouse for 6 days, lower leaf epidermis removed and stored in liquid nitrogen. | 3109 | 3730 | 486 | 723 | 3007 | |
HQ | Barke | total immature inflorescence (1 cm), plants taken from the field at 7:30 am, sunny day, isolation of inflorescences during morning (overperiod of 3 hours) | 654 | 654 | 570 | 0 | 654 | |
HR | Barke | root(grown on filter paper at 250C for 48 hours in petridish, 1-1.5 cm in length) | 334 | 541 | 428 | 239 | 302 | |
HS | Barke | embryo + scutellum (0-16 hours after imbibition) | 4826 | 7086 | 448 | 2536 | 4550 | |
HT | Barke | endosperm (0-16 hours after imbibition) | 4926 | 6834 | 535 | 2052 | 4782 | |
HU | Barke | germinating seeds, 16-48h | 4633 | 6906 | 519 | 2503 | 4403 | |
HV | Barke | germinating seeds, 48-96h | 4463 | 6708 | 496 | 2535 | 4173 | |
HW | Barke | Roots were grown for two days on filter paper at room temperature | 3020 | 5407 | 526 | 2643 | 2764 | |
HX | Barke | apex (3-5 mm in size) | 4989 | 7899 | 554 | 3266 | 4633 | |
HY | Barke | developing caryopsis, 0-15 DAF (days after flowering) | 3517 | 6319 | 552 | 3070 | 3249 | |
HZ | Barke | pericarp 0-7 DAF (days after flowering), maternal tissue | 9780 | 10048 | 458 | 296 | 9752 | |
RUS | Barke | HA: 0-7 DAF (days after flowering), filial seed part; HZ: 0-7 DAF, maternal seed part; HB: 8-15 DAF, whole caryopsis; HF: 16-25 DAF, whole caryopsis | 10594 | 10766 | 573 | 9483 | 1283 | |