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cDNA library details

This table displays detailed information about the cDNA library SSBT .

Properties

AuthorHeike Nierig, Sophia Biemelt, Uwe Sonnewald
CultivarSolara
Descriptionsprouting tuber buds were excised, PolyA-RNA was isolated and used to create a cDNA-library by means of the SMART cDNA library construction Kit (CLONTECH). cDNA Fragments were inserted into the vector Lambda TripleEx Sfi I. Subsequently, plasmids were obtained by in vivo excision according to manufacturer's instruction.
Entry date01-NOV-2002
LaboratoryMEP, MPP
Laboratory hostE. coli BM25.8
Number of plates12
Plate size384
Restriction site ISfi IA
Restriction site IISfi IB
Tissuepotato tubers after ca. 3 months of storage, sprouting buds (ca. 2mm length)
VectorpTriplEx2
cDNA LIBRARY IDSSBT

Features

1,661Number of ESTs
1,661Number of clones
101Minimum EST length
700Maximum EST length
383Average EST length
03´ ESTs
1,6615´ ESTs

Clones/EST ratio

EST direction ratio

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