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cDNA library details

This table displays detailed information about the cDNA library SDBT .

Properties

AuthorHeike Nierig, Sophia Biemelt, Uwe Sonnewald
CultivarSolara
Descriptiondormant tuber buds were excised, PolyA-RNA was isolated and used to create a cDNA-library by means of the SMART cDNA library construction Kit (CLONTECH). CDNA Fragments were inserted into the vector Lambda TripleEx Sfi I. Subsequently, plasmids were obtained by in vivo excision according to manufacturer's instruction.
Entry date01-NOV-2002
LaboratoryMEP, MPP
Laboratory hostE. coli BM25.8
Number of plates12
Plate size384
Restriction site ISfi IA
Restriction site IISfi IB
Tissuepotato tuber, dormant buds
VectorpTriplEx2
cDNA LIBRARY IDSDBT

Features

561Number of ESTs
561Number of clones
101Minimum EST length
700Maximum EST length
426Average EST length
03´ ESTs
5615´ ESTs

Clones/EST ratio

EST direction ratio

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