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cDNA library details

This table displays detailed information about the cDNA library SDBN .

Properties

AuthorHeike Nierig, Sophia Biemelt, Uwe Sonnewald
CultivarSolara
Descriptionsprouting tuber buds were excised from potato tubers, PolyA-RNA was isolated and used to create a cDNA-library by means of by means of the "SMART cDNA library construction Kit" (CLONTECH). Normalisation was performed essentially as described by Ko (1990, Nucl. Acid Res. 16, 9877) with some modification of Kohchi et al. (1995, Plant J. 8, 771-776). Normalised cDNAs were digested with NotI and cloned into NotI digested pCRblunt vector.
Entry date01-MAR-2004
LaboratoryMEP, MPP
Laboratory hostE. coli XL1-blue
Number of plates15
Plate size384
Restriction site INotI (GCGGCCGC)
Restriction site IINotI (GCGGCCGC)
Tissuepotato tuber, dormant buds (taken from freshly harvested tubers)
VectorpCR-Blunt (Invitrogen)
cDNA LIBRARY IDSDBN

Features

2,028Number of ESTs
2,028Number of clones
104Minimum EST length
700Maximum EST length
496Average EST length
2,0283´ ESTs
05´ ESTs

Clones/EST ratio

EST direction ratio

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