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cDNA library details

This table displays detailed information about the cDNA library HZ .

Properties

AuthorHangning Zhang, Ulrich Wobus, Volodymyr Radchuk, Winfriede Weschke
CultivarBarke
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp
Entry date01-JAN-2002
Laboratory*
Laboratory hostXL10_Gold
Number of plates72
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
Tissuepericarp 0-7 DAF (days after flowering), maternal tissue
VectorpBluescript SK+
cDNA LIBRARY IDHZ

Features

10,048Number of ESTs
9,780Number of clones
100Minimum EST length
700Maximum EST length
458Average EST length
2963´ ESTs
9,7525´ ESTs

Clones/EST ratio

EST direction ratio

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