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cDNA library details

This table displays detailed information about the cDNA library HW .

Properties

AuthorAndreas Graner, Klaus-Peter Pleissner, Winfriede Weschke, Wolfgang Michalek
CultivarBarke
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb
Entry date01-JAN-2000
Laboratory*
Laboratory hostXLOLR
Number of plates63
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
TissueRoots were grown for two days on filter paper at room temperature
Vectorlambda ZAP Express, subsequent in vivo excision resulted in plasmid pBK-CMV
cDNA LIBRARY IDHW

Features

5,407Number of ESTs
3,020Number of clones
16Minimum EST length
700Maximum EST length
526Average EST length
2,6433´ ESTs
2,7645´ ESTs

Clones/EST ratio

EST direction ratio

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