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cDNA library details

This table displays detailed information about the cDNA library HU .

Properties

AuthorAndreas Graner, Elena Potokina, Hangning Zhang, Nils Stein, Winfriede Weschke, Wolfgang Michalek
CultivarBarke
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1.2 kb
Entry date01-JAN-2002
Laboratory*
Laboratory hostXL10_Gold
Number of plates74
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
Tissuegerminating seeds, 16-48h
VectorpBluescript SK+
cDNA LIBRARY IDHU

Features

6,906Number of ESTs
4,633Number of clones
108Minimum EST length
700Maximum EST length
519Average EST length
2,5033´ ESTs
4,4035´ ESTs

Clones/EST ratio

EST direction ratio

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