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cDNA library details

This table displays detailed information about the cDNA library HS .

Properties

AuthorAndreas Graner, Elena Potokina, Hangning Zhang, Nils Stein, Winfriede Weschke, Wolfgang Michalek
CultivarBarke
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
Entry date01-JAN-2002
Laboratory*
Laboratory hostXL10_Gold
Number of plates66
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
Tissueembryo + scutellum (0-16 hours after imbibition)
VectorpBluescript SK+
cDNA LIBRARY IDHS

Features

7,086Number of ESTs
4,826Number of clones
106Minimum EST length
700Maximum EST length
448Average EST length
2,5363´ ESTs
4,5505´ ESTs

Clones/EST ratio

EST direction ratio

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