New

cDNA library details

This table displays detailed information about the cDNA library HR .

Properties

AuthorAndreas Graner, Hangning Zhang, Nils Stein, Volodymyr Radchuk, Winfriede Weschke, Wolfgang Michalek
CultivarBarke
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 800 bp
Entry date01-JAN-2002
Laboratory*
Laboratory hostXL10_Gold
Number of plates82
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
Tissueroot(grown on filter paper at 250C for 48 hours in petridish, 1-1.5 cm in length)
VectorpBluescript SK+
cDNA LIBRARY IDHR

Features

541Number of ESTs
334Number of clones
111Minimum EST length
638Maximum EST length
428Average EST length
2393´ ESTs
3025´ ESTs

Clones/EST ratio

EST direction ratio

© Copyright 2003 - 2021 , IPK Gatersleben