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cDNA library details

This table displays detailed information about the cDNA library HJ .

Properties

AuthorAndreas Graner, Andrew Milligan, Hangning Zhang, Nils Stein, Peter Langridge, Rajeev Kumar Varshney
CultivarSloop
DescriptionDue to the cloning system used Blue/white selection for recombinants is not 100% reliable. Average insert size is 1.2 kb.
Entry date01-JAN-2002
Laboratory*
Laboratory hostXL-10 Gold
Number of plates12
Plate size384
Restriction site IcDNAs were directionally cloned using asymmetric SfiI sites
Restriction site IIcDNAs were directionally cloned using asymmetric SfiI sites
Tissueembryos isolated 8 days after pollination, corresponding to the globular stage of embryo development.
Vectorlambda TripEx2 (Clontech)
cDNA LIBRARY IDHJ

Features

197Number of ESTs
197Number of clones
126Minimum EST length
690Maximum EST length
533Average EST length
03´ ESTs
1975´ ESTs

Clones/EST ratio

EST direction ratio

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