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cDNA library details

This table displays detailed information about the cDNA library HA .

Properties

AuthorHangning Zhang, Ulrich Wobus, Winfriede Weschke
CultivarBarke
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb
Entry date01-JAN-2002
Laboratory*
Laboratory hostXL10_Gold
Number of plates70
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
Tissueembryosac, 0-7 DAF (days after flowering), filial tissue
VectorpBluescript SK+
cDNA LIBRARY IDHA

Features

12,785Number of ESTs
10,055Number of clones
102Minimum EST length
700Maximum EST length
487Average EST length
3,0223´ ESTs
9,7635´ ESTs

Clones/EST ratio

EST direction ratio

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