cDNA library details

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This table displays detailed information about the cDNA library GBN .

Library property	Value
Author	Carin Jansen, Heike Nierig, Karl-Heinz Kogel, Patrick Schaefer, Sophia Biemelt, Uwe Sonnewald
Cultivar	backcross: Ingrid mlo5 and Ingrid MLG
Description	PolyA-RNA was isolated from leaf epidermis and used to create a cDNA-library using the Time Saver cDNA-Synthesis Kit (Amersham). Normalisation was performed essentially as described by Ko (1990, Nucl. Acid Res. 16, 9877) with some modification of Kohchi et al. (1995, Plant J. 8, 771-776). Normalised cDNAs were digested with NotI and cloned into NotI digested pCRblunt vector. (GABI-Agrotech Project)
Entry date	01-MAR-2002
Laboratory	MPP, MEP, IPK; IPAZ (Institute of Phytopathology and Applied Zoology, University Giessen)
Laboratory host	XL1-Blue
Number of plates	16
Plate size	384
Restriction site I	NotI
Restriction site II	NotI
Tissue	leaf epidermis; seedlings were grown at 18 degr. C, 60 % rel. humidity, and a photoperiod of 16 h (100 microE * s-1 * m-2). A. 8 days, seedl. were treated with cuture filtrate of Bipolaris sorokiniana. Leaf epid. was harv. 6, 12, 24, 48 hpt
Vector	pCR blunt (Invitrogen)
cDNA LIBRARY ID	GBN