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cDNA library details

This table displays detailed information about the cDNA library GAN .

Properties

AuthorCarin Jansen, Heike Nierig, Karl-Heinz Kogel, Patrick Schaefer, Sophia Biemelt, Uwe Sonnewald
CultivarIngrid WT
DescriptionPolyA-RNA was isolated from leaf epidermis and used to create a cDNA-library using the Time Saver cDNA-Synthesis Kit (Amersham). Normalisation was performed essentially as described by Ko (1990, Nucl. Acid Res. 16, 9877) with some modification of Kohchi et al. (1995, Plant J. 8, 771-776). Normalised cDNAs were digested with NotI and cloned into NotI digested pCRblunt vector. (GABI-Agrotech Project)
Entry date01-MAR-2002
LaboratoryMPP, MEP, IPK; IPAZ (Institute of Phytopathology and Applied Zoology, University Giessen)
Laboratory hostXL1-Blue
Number of plates9
Plate size384
Restriction site INotI
Restriction site IINotI
Tissueleaf epidermis; seedlings were grown at 18 degr. C, 60 % rel. humidity, and a photoperiod of 16 h (100 microE * s-1 * m-2). A. 8 days, seedl. were treated with ASM (syn. BTH; 20 ppm soil drench). Leaf epid. was harv. 8, 24, 48, 72 hpt
VectorpCR blunt (Invitrogen)
cDNA LIBRARY IDGAN

Features

3,068Number of ESTs
3,068Number of clones
100Minimum EST length
700Maximum EST length
403Average EST length
2,0203´ ESTs
1,0485´ ESTs

Clones/EST ratio

EST direction ratio

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