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cDNA library details

This table displays detailed information about the cDNA library STDB .

Properties

AuthorHeike Nierig, Sophia Biemelt, Uwe Sonnewald
CultivarSolara
Descriptiondormant tuber buds were excised, PolyA-RNA was isolated and used to create a cDNA-library using the Time Saver(TM) cDNA-Synthesis Kit (Amersham) and inserted into the vector Lambda ZAPII vector (Stratagene). Average insert size is 750bp
Entry date01-JAN-2002
LaboratoryMEP, MPP
Laboratory hostXL1-Blue MRF
Number of plates12
Plate size384
Restriction site IEcoRI /NotI
Restriction site IIEcoRI /NotI
Tissuepotato tuber, dormant buds
VectorpBluescript SK-
cDNA LIBRARY IDSTDB

Features

1,455Number of ESTs
1,455Number of clones
50Minimum EST length
700Maximum EST length
494Average EST length
1,4553´ ESTs
05´ ESTs

Clones/EST ratio

EST direction ratio

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