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cDNA library details

This table displays detailed information about the cDNA library SSBN .

Properties

AuthorHeike Nierig, Sophia Biemelt, Uwe Sonnewald
CultivarSolara
Descriptionsprouting tuber buds were excised from potato tubers, PolyA-RNA was isolated and used to create a cDNA-library by means of by means of the "SMART cDNA library construction Kit" (CLONTECH). Normalisation was performed essentially as described by Ko (1990, Nucl. Acid Res. 16, 9877) with some modification of Kohchi et al. (1995, Plant J. 8, 771-776). Normalised cDNAs were digested with NotI and cloned into NotI digested pCRblunt vector.
Entry date01-JUL-2002
LaboratoryMEP, MPP
Laboratory hostE. coli XL1-blue
Number of plates24
Plate size384
Restriction site INotI (GCGGCCGC)
Restriction site IINotI (GCGGCCGC)
Tissuepotato tubers after ca. 3 months of storage, sprouting buds (ca. 2mm length)
VectorpCR-Blunt (Invitrogen)
cDNA LIBRARY IDSSBN

Features

1,128Number of ESTs
1,128Number of clones
135Minimum EST length
700Maximum EST length
561Average EST length
1,1283´ ESTs
05´ ESTs

Clones/EST ratio

EST direction ratio

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