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cDNA library details

This table displays detailed information about the cDNA library RUS .

Properties

AuthorAndreas Graner, Nese Sreenivasulu, Ulrich Wobus, Volodymyr Radchuk, Winfriede Weschke
CultivarBarke
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is > 1 kb
Entry date01-APR-2003
LaboratoryAG GW, AG MOM, IPK Gatersleben, Corrensstr. 3, 06466 Gatersleben, Germany
Laboratory hostXL10-Gold
Number of plates28
Plate size96
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
TissueHA: 0-7 DAF (days after flowering), filial seed part; HZ: 0-7 DAF, maternal seed part; HB: 8-15 DAF, whole caryopsis; HF: 16-25 DAF, whole caryopsis
VectorpBluescript SK+
cDNA LIBRARY IDRUS

Features

10,766Number of ESTs
10,594Number of clones
100Minimum EST length
700Maximum EST length
573Average EST length
9,4833´ ESTs
1,2835´ ESTs

Clones/EST ratio

EST direction ratio

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