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cDNA library details

This table displays detailed information about the cDNA library PHBS .

Properties

AuthorHeike Nierig, Mohammad-Reza Hajirezaei
CultivarPetunia hybrida
DescriptionPetunia cutting base was harvested at different times during adventitious root development (ARD). PolyA(+)mRNA was isolated and used to create a cDNA library by means of the ¿Timer saver cDNA Library Kit¿ from Amersham Biosciences. Normalization was carried out essentially as described by Ko (1990, Nucl. Acid Res. 16, 9877) with some modification of Kohchi et al. (1995, Plant J. 8, 771-776). Normalised cDNAs were digested with NotI and cloned into NotI digested pCRblunt vector.
Entry date01-JUN-2007
LaboratoryMZB, MPP
Laboratory hostE.coli Top 10 cells
Number of plates15
Plate size384
Restriction site INotI (GCGGCCGC)
Restriction site IINotI (GCGGCCGC)
TissuePetunia cutting base 0.5 cm, time kinetic during adventitious root development (ARD) from 0 to 8 days
VectorPCR blunt (Invitrogen)
cDNA LIBRARY IDPHBS

Features

4,700Number of ESTs
4,700Number of clones
101Minimum EST length
700Maximum EST length
495Average EST length
4,7003´ ESTs
05´ ESTs

Clones/EST ratio

EST direction ratio

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