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cDNA library details

This table displays detailed information about the cDNA library HY .

Properties

AuthorAndreas Graner, Klaus-Peter Pleissner, Winfriede Weschke, Wolfgang Michalek
CultivarBarke
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is >1 kb
Entry date01-JAN-2000
Laboratory*
Laboratory hostXLOLR
Number of plates62
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
Tissuedeveloping caryopsis, 0-15 DAF (days after flowering)
Vectorlambda ZAP Express, subsequent in vivo excision resulted in plasmid pBK-CMV
cDNA LIBRARY IDHY

Features

6,319Number of ESTs
3,517Number of clones
61Minimum EST length
701Maximum EST length
552Average EST length
3,0703´ ESTs
3,2495´ ESTs

Clones/EST ratio

EST direction ratio

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