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cDNA library details

This table displays detailed information about the cDNA library HX .

Properties

AuthorAndreas Graner, Hangning Zhang, Nils Stein, Volodymyr Radchuk, Winfriede Weschke, Wolfgang Michalek
CultivarBarke
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
Entry date01-JAN-2002
Laboratory*
Laboratory hostXL10_Gold
Number of plates43
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
Tissueapex (3-5 mm in size)
VectorpBluescript SK+
cDNA LIBRARY IDHX

Features

7,899Number of ESTs
4,989Number of clones
100Minimum EST length
700Maximum EST length
554Average EST length
3,2663´ ESTs
4,6335´ ESTs

Clones/EST ratio

EST direction ratio

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