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cDNA library details

This table displays detailed information about the cDNA library HO .

Properties

AuthorPatrick Schweizer, Uwe Zierold
CultivarIngrid BC mlo5
DescriptionApproximately 5 % of the clones correspond to cDNA from the fungi B. g. hordei and tritici, respectively. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1,2 kb
Entry date01-JAN-2002
Laboratory*
Laboratory hostXL10_Gold
Number of plates38
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
TissueEpidermis stripped 6 and 24 hours post inoculation from seven day old plants inoculated with Blumeria graminis hordei or B. g. tritici
VectorpBluescript SK+
cDNA LIBRARY IDHO

Features

22,827Number of ESTs
13,143Number of clones
100Minimum EST length
770Maximum EST length
562Average EST length
10,7113´ ESTs
12,1165´ ESTs

Clones/EST ratio

EST direction ratio

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