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cDNA library details

This table displays detailed information about the cDNA library HG .

Properties

AuthorAndreas Graner, Hangning Zhang, Nils Stein, Winfriede Weschke, Wolfgang Michalek
CultivarBarke
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 800 bp
Entry date01-JAN-2002
Laboratory*
Laboratory hostXL10_Gold
Number of plates72
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
Tissuegreen leaves
VectorpBluescript SK+
cDNA LIBRARY IDHG

Features

565Number of ESTs
344Number of clones
107Minimum EST length
647Maximum EST length
467Average EST length
2373´ ESTs
3285´ ESTs

Clones/EST ratio

EST direction ratio

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