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cDNA library details

This table displays detailed information about the cDNA library HF .

Properties

AuthorHangning Zhang, Ulrich Wobus, Volodymyr Radchuk, Winfriede Weschke
CultivarBarke
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 940 bp
Entry date01-JAN-2002
Laboratory*
Laboratory hostXL10_Gold
Number of plates70
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
Tissuedeveloping caryopsis, 16-25 DAF (days after flowering)
VectorpBluescript SK+
cDNA LIBRARY IDHF

Features

7,692Number of ESTs
7,420Number of clones
111Minimum EST length
700Maximum EST length
543Average EST length
2803´ ESTs
7,4125´ ESTs

Clones/EST ratio

EST direction ratio

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