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cDNA library details

This table displays detailed information about the cDNA library HE .

Properties

AuthorAndreas Graner, Hangning Zhang, Nils Stein, Volodymyr Radchuk, Winfriede Weschke, Wolfgang Michalek
CultivarBarke
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 900 bp
Entry date01-JAN-2002
Laboratory*
Laboratory hostXL10_Gold
Number of plates60
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
Tissueetiolated leaves (grown for 6 days in growth chamber, harvested at daylight conditions)
VectorpBluescript SK+
cDNA LIBRARY IDHE

Features

551Number of ESTs
336Number of clones
115Minimum EST length
700Maximum EST length
427Average EST length
2423´ ESTs
3095´ ESTs

Clones/EST ratio

EST direction ratio

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