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cDNA library details

This table displays detailed information about the cDNA library HDP .

Properties

AuthorAndreas Graner, Elena Potokina, Hangning Zhang, Nils Stein, Winfriede Weschke
CultivarBarke
DescriptionThe RNA samples were pooled and subtracted against roughly 20,000 cDNA clones representing a unigene set based on sequencing resultsfrom the 22 IPK H* libraries. The insert can be relesed by EcoRI/SmaI or EcoRI/NotI. The library was custom made by the company GATC, Konstanz, Germany
Entry date02-NOV-2002
LaboratoryAG MOM, AG GW, IPK Gatersleben, Corrensstr. 3, 06466 Gatersleben, Germany
Laboratory hostDH10B
Number of plates100
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IISma I (3'-end of cDNA)
Tissuepooled sample of 15 vegetative and generative barley tissues. PCR depleted against a ~20.000 unigene set and normalized. Manufactured by company GATC.
VectorpBluescript SK+
cDNA LIBRARY IDHDP

Features

29,397Number of ESTs
15,258Number of clones
100Minimum EST length
700Maximum EST length
546Average EST length
14,5883´ ESTs
14,8095´ ESTs

Clones/EST ratio

EST direction ratio

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