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cDNA library details

This table displays detailed information about the cDNA library HB .

Properties

AuthorHangning Zhang, Ulrich Wobus, Volodymyr Radchuk, Winfriede Weschke
CultivarBarke
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
Entry date01-JAN-2002
Laboratory*
Laboratory hostXL10_Gold
Number of plates56
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
Tissuedeveloping caryopsis, 8-15 DAF (days after flowering)
VectorpBluescript SK+
cDNA LIBRARY IDHB

Features

10,275Number of ESTs
10,063Number of clones
102Minimum EST length
700Maximum EST length
472Average EST length
2453´ ESTs
10,0305´ ESTs

Clones/EST ratio

EST direction ratio

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