New

cDNA library details

This table displays detailed information about the cDNA library GW .

Properties

AuthorCarin Jansen, Heike Nierig, Karl-Heinz Kogel, Sophia Biemelt, Uwe Sonnewald
CultivarNickel and Carola
DescriptionPolyA-RNA was isolated from water treated roots and used to create a cDNA-library by means of the SMART cDNA library construction Kit (CLONTECH). CDNA Fragments were inserted into the vector Lambda TripleEx Sfi I. Subsequently, plasmids were obtained by in vivo excision according to manufacturer's instruction. (GABI-Agrotech project)
Entry date01-OCT-2002
LaboratoryMPP, MEP, IPK; IPAZ (Institute of Phytopathology and Applied Zoology, University Giessen)
Laboratory hostE. coli BM25.8
Number of plates14
Plate size384
Restriction site ISfi IA
Restriction site IISfi IB
Tissueroots (Control); Kernels were germinated and subsequently grown in a mixture of SERAMIS and OIL DRI soil at 20 degr. C, 16h light and 50-60% rel. humidity, Roots were harvested after 7 days
VectorpTriplEx2
cDNA LIBRARY IDGW

Features

847Number of ESTs
847Number of clones
101Minimum EST length
700Maximum EST length
437Average EST length
03´ ESTs
8475´ ESTs

Clones/EST ratio

EST direction ratio

© Copyright 2003 - 2021 , IPK Gatersleben