cDNA library details

New

This table displays detailed information about the cDNA library GPN .

Library property	Value
Author	Angelika Felk, Heike Nierig, Sophia Biemelt, Uwe Sonnewald, Wilhelm Schaefer
Cultivar	Pasadena (susceptible cultivar)
Description	Each second spikelet of barley was infected with Fusarium graminearum (strain FG2311, isolated from wheat, 1992, Hohenheim, Germany) by means of micropipette using 5 µl of conidia suspension (500 conidia /µl). The infected plants were incubated 4d by 16 h light and 20°C and infected spikes were incubated the first 48 h by 100% rel. humidity for establishing of infection. PolyA-RNA was isolated from barley spikelets and used to create a cDNA-library by means of the "SMART cDNA library construction Kit" (CLONTECH). Normalisation was performed essentially as described by Ko (1990, Nucl. Acid Res. 16, 9877) with some modification of Kohchi et al. (1995, Plant J. 8, 771-776). Normalised cDNAs were digested with NotI and cloned into NotI digested pCRblunt vector. (GABI-Agrotech Project)
Entry date	01-FEB-2004
Laboratory	MPP, MEP, IPK; Institut für Allgemeine Botanik, Uni Hamburg
Laboratory host	E. coli XL1-blue
Number of plates	0
Plate size	384
Restriction site I	NotI (GCGGCCGC)
Restriction site II	NotI (GCGGCCGC)
Tissue	with Fusarium graminearum infected spikelets
Vector	pCR-blunt (Invitrogen)
cDNA LIBRARY ID	GPN