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cDNA library details

This table displays detailed information about the cDNA library GNW .

Properties

AuthorCarin Jansen, Heike Nierig, Karl-Heinz Kogel, Sophia Biemelt, Uwe Sonnewald
CultivarNickel, susceptible
DescriptionPolyA-RNA was isolated from water treated roots and used to create a cDNA-library by means of the SMART cDNA library construction Kit (CLONTECH). CDNA Fragments were inserted into the vector Lambda TripleEx Sfi I. Subsequently, plasmids were obtained by in vivo excision according to manufacturer's instruction. (GABI-Agrotech Project)
Entry date01-OCT-2002
LaboratoryMPP, MEP, IPK; IPAZ (Institute of Phytopathology and Applied Zoology, University Giessen)
Laboratory hostE.coli BM25.8
Number of plates9
Plate size384
Restriction site ISfi IA
Restriction site IISfi IB
Tissueroots, Seedlings were grown in a mixture of SERAMIS and OIL DRI soil at 20 degr. C, 16h light and 50-60% rel. humidity for 7 days, Roots were harvested 6, 24, 48, 72, and 96 hpi (hours post inoculation) with Fusarium culmorum KF 350
VectorpTriplEx2
cDNA LIBRARY IDGNW

Features

1,168Number of ESTs
1,168Number of clones
105Minimum EST length
667Maximum EST length
366Average EST length
03´ ESTs
1,1685´ ESTs

Clones/EST ratio

EST direction ratio

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