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cDNA library details

This table displays detailed information about the cDNA library HD .

Properties

Library property
Value
AuthorAndreas Graner, Hangning Zhang, Nils Stein, Volodymyr Radchuk, Winfriede Weschke, Wolfgang Michalek
CultivarGolden Promise
DescriptionDue to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI, PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb
Entry date01-JAN-2002
Laboratory*
Laboratory hostXL10_Gold
Number of plates70
Plate size384
Restriction site IEcoRI (5'-end of cDNA)
Restriction site IIXhoI (3'-end of cDNA)
Tissuecallus, 5-10mm in diameter
VectorpBluescript SK+
cDNA LIBRARY IDHD

Features

Value
Statistic
7,418Number of ESTs
4,972Number of clones
102Minimum EST length
700Maximum EST length
515Average EST length
2,9683´ ESTs
4,4505´ ESTs

Clones/EST ratio

EST direction ratio

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