cDNA library details

This table displays detailed information about the cDNA library GBN .


AuthorCarin Jansen, Heike Nierig, Karl-Heinz Kogel, Patrick Schaefer, Sophia Biemelt, Uwe Sonnewald
Cultivarbackcross: Ingrid mlo5 and Ingrid MLG
DescriptionPolyA-RNA was isolated from leaf epidermis and used to create a cDNA-library using the Time Saver cDNA-Synthesis Kit (Amersham). Normalisation was performed essentially as described by Ko (1990, Nucl. Acid Res. 16, 9877) with some modification of Kohchi et al. (1995, Plant J. 8, 771-776). Normalised cDNAs were digested with NotI and cloned into NotI digested pCRblunt vector. (GABI-Agrotech Project)
Entry date01-MAR-2002
LaboratoryMPP, MEP, IPK; IPAZ (Institute of Phytopathology and Applied Zoology, University Giessen)
Laboratory hostXL1-Blue
Number of plates16
Plate size384
Restriction site INotI
Restriction site IINotI
Tissueleaf epidermis; seedlings were grown at 18 degr. C, 60 % rel. humidity, and a photoperiod of 16 h (100 microE * s-1 * m-2). A. 8 days, seedl. were treated with cuture filtrate of Bipolaris sorokiniana. Leaf epid. was harv. 6, 12, 24, 48 hpt
VectorpCR blunt (Invitrogen)


3,058Number of ESTs
3,058Number of clones
101Minimum EST length
700Maximum EST length
377Average EST length
2,3253´ ESTs
7335´ ESTs

Clones/EST ratio

EST direction ratio

© Copyright 2003 - 2021 , IPK Gatersleben